Mapping of VSG similarities in Trypanosoma brucei
نویسندگان
چکیده
منابع مشابه
Analysis of Trypanosoma brucei vsg expression site switching in vitro.
Trypanosoma brucei can undergo antigenic variation by switching between distinct telomeric variant surface glycoprotein gene (vsg) expression sites (ESs) or by replacing the active vsg. DNA rearrangements have often been associated with ES switching, but it is unclear if such rearrangements are necessary or whether ES inactivation always accompanies ES activation. To explore these issues, we de...
متن کاملVSG switching in Trypanosoma brucei: antigenic variation analysed using RNAi in the absence of immune selection.
Trypanosoma brucei relies on antigenic variation of its variant surface glycoprotein (VSG) coat for survival. We show that VSG switching can be efficiently studied in vitro using VSG RNAi in place of an immune system to select for switch variants. Contrary to models predicting an instant switch after inhibition of VSG synthesis, switching was not induced by VSG RNAi and occurred at a rate of 10...
متن کاملMasters of Disguise: Antigenic Variation and the VSG Coat in Trypanosoma brucei
VSG stands for variant surface glycoprotein, the major surface component of the protozoan parasite Trypanosoma brucei while it exists in the blood and tissues of its mammalian host. Transmitted by the bite of the tsetse (Glossina spp.), T. brucei infects mammals in sub-Saharan Africa. Two subspecies, T. brucei gambiense and T. b. rhodesiense, infect humans, causing human African trypanosomiasis...
متن کاملNuclear repositioning of the VSG promoter during developmental silencing in Trypanosoma brucei
Interphase nuclear repositioning of chromosomes has been implicated in the epigenetic regulation of RNA polymerase (pol) II transcription. However, little is known about the nuclear position-dependent regulation of RNA pol I-transcribed loci. Trypanosoma brucei is an excellent model system to address this question because its two main surface protein genes, procyclin and variant surface glycopr...
متن کاملLocalization of the modified base J in telomeric VSG gene expression sites of Trypanosoma brucei.
African trypanosomes such as Trypanosoma brucei undergo antigenic variation in the bloodstream of their mammalian hosts by regularly changing the variant surface glycoprotein (VSG) gene expressed. The transcribed VSG gene is invariably located in a telomeric expression site. There are multiple expression sites and one way to change the VSG gene expressed is by activating a new site and inactiva...
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ژورنال
عنوان ژورنال: Molecular and Biochemical Parasitology
سال: 2012
ISSN: 0166-6851
DOI: 10.1016/j.molbiopara.2011.10.011